TY - JOUR
T1 - Sequence analysis of cDNAs encoding precursors of starfish asterosaps
AU - Matsumoto, Midori
AU - Briones, Annabelle V.
AU - Nishigaki, Takuya
AU - Hoshi, Motonori
PY - 1999
Y1 - 1999
N2 - To understand how starfish sperm activating peptides (asterosaps) are synthesized in the ovary, we cloned cDNAs encoding asterosaps and elucidated their nucleotide sequences. The mRNA encoding asterosaps was synthesized only in the oocytes, but not in the follicle cells, and the length was 3.7 kb. The cDNA clones contained multiple isoforms of asterosaps. We assume that asterosap precursors are large prepoly-peptide chains with an unusual 'rosary-type' structure made of 10 successive similar stretches of 51-55 residues. Each stretch finishes with a 'spacer' of 17-21 residues immediately followed by the sequence of one asterosap isoform. The N-terminal of this precursor has 19-21 successive glutamine-rich repeating units. Maturation of the precursor may require endopeptidases that cleave both C- and N-sites of lysine-arginine.
AB - To understand how starfish sperm activating peptides (asterosaps) are synthesized in the ovary, we cloned cDNAs encoding asterosaps and elucidated their nucleotide sequences. The mRNA encoding asterosaps was synthesized only in the oocytes, but not in the follicle cells, and the length was 3.7 kb. The cDNA clones contained multiple isoforms of asterosaps. We assume that asterosap precursors are large prepoly-peptide chains with an unusual 'rosary-type' structure made of 10 successive similar stretches of 51-55 residues. Each stretch finishes with a 'spacer' of 17-21 residues immediately followed by the sequence of one asterosap isoform. The N-terminal of this precursor has 19-21 successive glutamine-rich repeating units. Maturation of the precursor may require endopeptidases that cleave both C- and N-sites of lysine-arginine.
KW - Acrosome reaction
KW - Invertebrate fertilization
KW - Precursor
KW - Sperm activating peptide
KW - Starfish
KW - cDNA cloning
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U2 - 10.1002/(SICI)1520-6408(1999)25:2<130::AID-DVG7>3.0.CO;2-8
DO - 10.1002/(SICI)1520-6408(1999)25:2<130::AID-DVG7>3.0.CO;2-8
M3 - Article
C2 - 10440847
AN - SCOPUS:0032838931
VL - 25
SP - 130
EP - 136
JO - Genesis
JF - Genesis
SN - 1526-954X
IS - 2
ER -