Sequence analysis of cDNAs encoding precursors of starfish asterosaps

Midori Matsumoto, Annabelle V. Briones, Takuya Nishigaki, Motonori Hoshi

研究成果: Article査読

17 被引用数 (Scopus)

抄録

To understand how starfish sperm activating peptides (asterosaps) are synthesized in the ovary, we cloned cDNAs encoding asterosaps and elucidated their nucleotide sequences. The mRNA encoding asterosaps was synthesized only in the oocytes, but not in the follicle cells, and the length was 3.7 kb. The cDNA clones contained multiple isoforms of asterosaps. We assume that asterosap precursors are large prepoly-peptide chains with an unusual 'rosary-type' structure made of 10 successive similar stretches of 51-55 residues. Each stretch finishes with a 'spacer' of 17-21 residues immediately followed by the sequence of one asterosap isoform. The N-terminal of this precursor has 19-21 successive glutamine-rich repeating units. Maturation of the precursor may require endopeptidases that cleave both C- and N-sites of lysine-arginine.

本文言語English
ページ(範囲)130-136
ページ数7
ジャーナルDevelopmental Genetics
25
2
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学
  • 細胞生物学

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