To understand how starfish sperm activating peptides (asterosaps) are synthesized in the ovary, we cloned cDNAs encoding asterosaps and elucidated their nucleotide sequences. The mRNA encoding asterosaps was synthesized only in the oocytes, but not in the follicle cells, and the length was 3.7 kb. The cDNA clones contained multiple isoforms of asterosaps. We assume that asterosap precursors are large prepoly-peptide chains with an unusual 'rosary-type' structure made of 10 successive similar stretches of 51-55 residues. Each stretch finishes with a 'spacer' of 17-21 residues immediately followed by the sequence of one asterosap isoform. The N-terminal of this precursor has 19-21 successive glutamine-rich repeating units. Maturation of the precursor may require endopeptidases that cleave both C- and N-sites of lysine-arginine.
|出版ステータス||Published - 1999|
ASJC Scopus subject areas