抄録
Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 180-182 |
| ページ数 | 3 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 76 |
| 号 | 1 |
| DOI | |
| 出版ステータス | Published - 2012 |
ASJC Scopus subject areas
- バイオテクノロジー
- 分析化学
- 生化学
- 応用微生物学とバイオテクノロジー
- 分子生物学
- 有機化学