Sequential production of various types of asymmetric lipid vesicles using pulse jet flow

Masahide Gotanda, Kaki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

This paper describes a method of sequential formation of asymmetric lipid vesicles, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by Droplet Split-and-Contact Method. In our previous work, we generated asymmetric lipid vesicles by deforming an asymmetric planar lipid bilayer using a pulsed jet flow. However, it was difficult to create various types or large amounts of the asymmetric lipid vesicles in a single double-well device. In this study, asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with a single device in a high-throughput manner using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.

本文言語English
ホスト出版物のタイトルTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
出版社Institute of Electrical and Electronics Engineers Inc.
ページ572-574
ページ数3
ISBN(電子版)9781538627310
DOI
出版ステータスPublished - 2017 7 26
イベント19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
継続期間: 2017 6 182017 6 22

出版物シリーズ

名前TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
国/地域Taiwan, Province of China
CityKaohsiung
Period17/6/1817/6/22

ASJC Scopus subject areas

  • 化学的な安全衛生
  • 器械工学
  • 電子工学および電気工学

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