Serum miRNA–based Prediction of Axillary Lymph Node Metastasis in Breast Cancer

Sho Shiino, Juntaro Matsuzaki, Akihiko Shimomura, Junpei Kawauchi, Satoko Takizawa, Hiromi Sakamoto, Yoshiaki Aoki, Masayuki Yoshida, Kenji Tamura, Ken Kato, Takayuki Kinoshita, Yuko Kitagawa, Takahiro Ochiya

研究成果: Article

11 引用 (Scopus)

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Purpose: Sentinel lymph node biopsy (SLNB) is the gold-standard procedure for evaluating axillary lymph node (ALN) status in patients with breast cancer. However, the morbidity of SLNB is not negligible, and the procedure is invasive for patients without ALN metastasis. Here, we developed a diagnostic model for evaluating ALN status using a combination of serum miRNAs and clinicopathologic factors as a novel less-invasive biomarker. Experimental Design: Preoperative serum samples were collected from patients who underwent surgery for primary breast cancer or breast benign diseases between 2008 and 2014. A total of 958 serum samples (921 cases of primary breast cancer, including 630 cases in the no ALN metastasis group and 291 cases in the ALN metastasis group, and 37 patients with benign breast diseases) were analyzed by miRNA microarray. Samples were randomly divided into training and test sets. Logistic LASSO regression analysis was used to construct diagnostic models in the training set, which were validated in the test set. Results: An optimal diagnostic model was identified using a combination of two miRNAs (miR-629-3p and miR-4710) and three clinicopathologic factors (T stage, lymphovascular invasion, and ultrasound findings), which showed a sensitivity of 0.88 (0.84–0.92), a specificity of 0.69 (0.61–0.76), an accuracy of 0.818, and an area under the receiver operating characteristic curve of 0.86 in the test set. Conclusions: Serum miRNA profiles may be useful for the diagnosis of ALN metastasis before surgery in a less-invasive manner than SLNB.

元の言語English
ページ(範囲)1817-1827
ページ数11
ジャーナルClinical Cancer Research
25
発行部数6
DOI
出版物ステータスPublished - 2019 1 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

フィンガープリント Serum miRNA–based Prediction of Axillary Lymph Node Metastasis in Breast Cancer' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Shiino, S., Matsuzaki, J., Shimomura, A., Kawauchi, J., Takizawa, S., Sakamoto, H., Aoki, Y., Yoshida, M., Tamura, K., Kato, K., Kinoshita, T., Kitagawa, Y., & Ochiya, T. (2019). Serum miRNA–based Prediction of Axillary Lymph Node Metastasis in Breast Cancer. Clinical Cancer Research, 25(6), 1817-1827. https://doi.org/10.1158/1078-0432.CCR-18-1414