@article{dbf9999f12344a6bb739db1982ccef08,
title = "Signal enhancement and Patterson-search phasing for high-spatial-resolution coherent X-ray diffraction imaging of biological objects",
abstract = "In this decade coherent X-ray diffraction imaging has been demonstrated to reveal internal structures of whole biological cells and organelles. However, the spatial resolution is limited to several tens of nanometers due to the poor scattering power of biological samples. The challenge is to recover correct phase information from experimental diffraction patterns that have a low signal-to-noise ratio and unmeasurable lowest-resolution data. Here, we propose a method to extend spatial resolution by enhancing diffraction signals and by robust phasing. The weak diffraction signals from biological objects are enhanced by interference with strong waves from dispersed colloidal gold particles. The positions of the gold particles determined by Patterson analysis serve as the initial phase, and this dramatically improves reliability and convergence of image reconstruction by iterative phase retrieval. A set of calculations based on current experiments demonstrates that resolution is improved by a factor of two or more.",
author = "Yuki Takayama and Saori Maki-Yonekura and Tomotaka Oroguchi and Masayoshi Nakasako and Koji Yonekura",
note = "Funding Information: We thank Masaki Yamamoto, Takaaki Hikima, Yuki Sekiguchi, Amane Kobayashi, Yukio Takahashi, Akihiro Suzuki and Takahiko Hoshi for the CXDI experiments, from which Y.T., S.M.-Y. and K.Y. obtained the basic idea for the approach described here. We are grateful to David B. McIntosh for his help in improving the manuscript. This work was supported by the RIKEN Special Postdoctoral Researchers Program and JSPS KAKENHI Grant Number 25891033 to Y.T., and in part by a grant for the strategic programs for R&D of RIKEN to K.Y. The parameters used in the calculation are based on XFEL-CXDI experiments with support by the MEXT X-ray Free Electron Laser Priority Strategy Program to M.N. under the approval of SACLA (2012A8005, 2012A8010, 2012B8037, 2013A8043 and 2013B8049).",
year = "2015",
month = jan,
day = "28",
doi = "10.1038/srep08074",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}