Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases

Toshiharu Furukawa, Masahiko Watanabe, Tetsuro Kubota, Suguru Kase, Shin Fujita, Takaaki Yamamoto, Yoshiro Saikawa, Tsong‐Hong ‐H Kuo, Hirokazu Tanino, Naoto Kurihara, Yukio Kawano, Kiyoshi Kawamoto, Akihiko Suto, Tatsuo Teramoto, Kyuya Ishibiki, Masaki Kitajima

研究成果: Article査読

13 被引用数 (Scopus)

抄録

The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)—Matrigel, laminin, fibronectin, and type IV collagen—was evaluated using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers. © 1993 Wiley‐Liss, Inc.

本文言語English
ページ(範囲)10-15
ページ数6
ジャーナルJournal of Surgical Oncology
53
1
DOI
出版ステータスPublished - 1993 5

ASJC Scopus subject areas

  • Surgery
  • Oncology

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