Significance of metastasis detected by molecular techniques in sentinel nodes of patients with gastrointestinal cancer.

Jun Ichi Matsuda, Yuko Kitagawa, Hirofumi Fujii, Makio Mukai, Katsuaki Dan, Tetsuro Kubota, Masahiko Watanabe, Soji Ozawa, Yoshihide Otani, Hirotoshi Hasegawa, Yoshimasa Shimizu, Koichiro Kumai, Atsushi Kubo, Masaki Kitajima

研究成果: Article査読

30 被引用数 (Scopus)

抄録

The clinical significance of micrometastasis in sentinel nodes (SNs) may differ in various organs. In particular, the prognostic value of SN micrometastases detected by reverse transcriptase-polymerase chain reaction (RT-PCR) is still controversial. We investigated the diagnostic and therapeutic significance of nodal molecular metastasis detected by nested RT-PCR for cytokeratin (CK) 19 mRNA in gastrointestinal cancer. In 51 cases with GI tract cancer treated by standard curative resection, SNs were identified by a radio-guided method. In 10 of 51 patients, 25 SNs and 3 non-SNs were histologically negative and RT-PCR positive. Three non-SNs with positive CK19 mRNA were randomly sampled from the same basin where histologically positive SNs were identified. Immunohistochemical analysis of six additional step sections obtained at 30- micro m intervals with use of an anticytokeratin antibody showed clearly recognizable histological metastases in 4 of 25 histologically negative/RT-PCR-positive SNs (16%). In one case of esophageal squamous cell carcinoma with nodal micrometastasis identified by CK19 RT-PCR, extranodal local recurrence in the SN basin (left supraclavicular basin) was observed 6 months postoperatively. These findings suggest that nodal micrometastasis detected by nested RT-PCR has some clinical significance in GI cancer. Molecular assessment of the SN may be a valuable tool to complement routine histological examination for GI cancers.

本文言語English
ページ(範囲)250S-4S
ジャーナルAnnals of surgical oncology : the official journal of the Society of Surgical Oncology
11
3 Suppl
DOI
出版ステータスPublished - 2004 3月

ASJC Scopus subject areas

  • 外科
  • 腫瘍学

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