Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord

Akimasa Yasuda, Osahiko Tsuji, Shinsuke Shibata, Satoshi Nori, Morito Takano, Yoshiomi Kobayashi, Yuichiro Takahashi, Kanehiro Fujiyoshi, Chikako Miyauchi Hara, Atsuhi Miyawaki, Hirotaka James Okano, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano

研究成果: Article査読

114 被引用数 (Scopus)

抄録

Previous reports of functional recovery from spinal cord injury (SCI) in rodents and monkeys after the delayed transplantation of neural stem/progenitor cells (NS/PCs) have raised hopes that stem cell therapy could be used to treat SCI in humans. More research is needed, however, to understand the mechanism of functional recovery. Oligodendrocytes derived from grafted NS/PCs remyelinate spared axons in the injured spinal cord. Here, we studied the extent of this remyelination's contribution to functional recovery following contusive SCI in mice. To isolate the effect of remyelination from other possible regenerative benefits of the grafted cells, NS/PCs obtained from myelin-deficient shiverer mutant mice (shi-NS/PCs) were used in this work alongside wild-type NS/PCs (wt-NS/PCs). shi-NS/PCs behaved like wt-NS/PCs in vitro and in vivo, with the exception of their myelinating potential. shi-NS/PC-derived oligodendrocytes did not express myelin basic protein in vitro and formed much thinner myelin sheaths in vivo compared with wt-NS/PC-derived oligodendrocytes. The transplantation of shi-NS/PCs promoted some locomotor and electrophysiological functional recovery but significantly less than that afforded by wt-NS/PCs. These findings establish the biological importance of remyelination by graft-derived cells for functional recovery after the transplantation of NS/PCs into the injured spinal cord.

本文言語English
ページ(範囲)1983-1994
ページ数12
ジャーナルStem Cells
29
12
DOI
出版ステータスPublished - 2011 12
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

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