Simple and rapid determination of serum carbapenem concentrations by high-performance liquid chromatography

Yuji Kurihara, Junko Kizu, Seiji Hori

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Although several high-performance liquid chromatography (HPLC) methods for the determination of serum concentrations of carbapenems have been reported, they are complicated and involve column switching. We established a simple and rapid HPLC method for the determination of serum concentrations of carbapenems, and this method is suitable for routine use in the clinical field. With our HPLC method, the serum concentrations of five commercially available carbapenems could be determined by changing only the methanol/phosphate-buffer ratio in the mobile phase. Serum levels of imipenem, panipenem, and meropenem in mice could be monitored when these carbapenems (20 mg/kg) were administered subcutaneously with cilastatin (20 mg/kg). These results suggest that our simple and rapid HPLC method for the determination of the serum concentrations of carbapenems is useful for pharmacokinetic/pharmacodynamic (PK/PD)-based determination of carbapenem dosage regimens.

本文言語English
ページ(範囲)30-34
ページ数5
ジャーナルJournal of Infection and Chemotherapy
14
1
DOI
出版ステータスPublished - 2008 2

ASJC Scopus subject areas

  • 微生物学(医療)
  • 薬理学(医学)
  • 感染症

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