Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes

Fumihiko Kawamoto, Sahoko Mizuno, Hisashi Fujioka, Nobuo Kumada, Etsuro Sugiyama, Tsutomu Takeuchi, Seiki Kobayashi, Motohiro Iseki, Minoru Yamada, Yoshitsugu Matsumoto, Tatsuya Tegoshi, Yukio Yoshida

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.

本文言語English
ページ(範囲)35-46
ページ数12
ジャーナルJapanese Journal of Medical Science and Biology
40
1
DOI
出版ステータスPublished - 1987
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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