Simultaneous administration of recombinant measles viruses expressing respiratory syncytial virus fusion (F) and nucleo (N) proteins induced humoral and cellular immune responses in cotton rats

Yoshiaki Yamaji, Akihito Sawada, Yosuke Yasui, Takashi Ito, Tetsuo Nakayama

研究成果: Article

抄録

We previously reported that recombinant measles virus expressing the respiratory syncytial virus (RSV) fusion protein (F), MVAIK/RSV/F, induced neutralizing antibodies against RSV, and those expressing RSV-NP (MVAIK/RSV/NP) and M2-1 (MVAIK/RSV/M2-1) induced RSV-specific CD8 + /IFN-γ + cells, but not neutralizing antibodies. In the present study, MVAIK/RSV/F and MVAIK/RSV/NP were simultaneously administered to cotton rats and immune responses and protective effects were compared with MVAIK/RSV/F alone. Sufficient neutralizing antibodies against RSV and RSV-specific CD8 + /IFN-γ + cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8 + /IFN-γ + increased in spleen cells obtained from the simultaneous immunization group in response to F and NP peptides. Higher numbers of CD8 + /IFN-γ + and CD4 + /IFN-γ + cells were detected in lung tissues from the simultaneous immunization group after the RSV challenge. No detectable RSV was recovered from lung homogenates in the immunized groups. Mild inflammatory reactions with the thickening of broncho-epithelial cells and the infiltration of inflammatory cells were observed in lung tissues obtained from cotton rats immunized with MVAIK/RSV/F alone after the RSV challenge. No inflammatory responses were observed after the RSV challenge in the simultaneous immunization groups. The present results indicate that combined administration with MVAIK/RSV/F and MVAIK/RSV/NP induces humoral and cellular immune responses and shows effective protection against RSV, suggesting the importance of cellular immunity.

元の言語English
記事番号27
ジャーナルVaccines
7
発行部数1
DOI
出版物ステータスPublished - 2019 3 1

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Sigmodontinae
Measles virus
Respiratory Syncytial Viruses
Humoral Immunity
Cellular Immunity
Proteins
Immunization
Neutralizing Antibodies
Lung

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Drug Discovery
  • Infectious Diseases
  • Pharmacology (medical)

これを引用

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title = "Simultaneous administration of recombinant measles viruses expressing respiratory syncytial virus fusion (F) and nucleo (N) proteins induced humoral and cellular immune responses in cotton rats",
abstract = "We previously reported that recombinant measles virus expressing the respiratory syncytial virus (RSV) fusion protein (F), MVAIK/RSV/F, induced neutralizing antibodies against RSV, and those expressing RSV-NP (MVAIK/RSV/NP) and M2-1 (MVAIK/RSV/M2-1) induced RSV-specific CD8 + /IFN-γ + cells, but not neutralizing antibodies. In the present study, MVAIK/RSV/F and MVAIK/RSV/NP were simultaneously administered to cotton rats and immune responses and protective effects were compared with MVAIK/RSV/F alone. Sufficient neutralizing antibodies against RSV and RSV-specific CD8 + /IFN-γ + cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8 + /IFN-γ + increased in spleen cells obtained from the simultaneous immunization group in response to F and NP peptides. Higher numbers of CD8 + /IFN-γ + and CD4 + /IFN-γ + cells were detected in lung tissues from the simultaneous immunization group after the RSV challenge. No detectable RSV was recovered from lung homogenates in the immunized groups. Mild inflammatory reactions with the thickening of broncho-epithelial cells and the infiltration of inflammatory cells were observed in lung tissues obtained from cotton rats immunized with MVAIK/RSV/F alone after the RSV challenge. No inflammatory responses were observed after the RSV challenge in the simultaneous immunization groups. The present results indicate that combined administration with MVAIK/RSV/F and MVAIK/RSV/NP induces humoral and cellular immune responses and shows effective protection against RSV, suggesting the importance of cellular immunity.",
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author = "Yoshiaki Yamaji and Akihito Sawada and Yosuke Yasui and Takashi Ito and Tetsuo Nakayama",
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TY - JOUR

T1 - Simultaneous administration of recombinant measles viruses expressing respiratory syncytial virus fusion (F) and nucleo (N) proteins induced humoral and cellular immune responses in cotton rats

AU - Yamaji, Yoshiaki

AU - Sawada, Akihito

AU - Yasui, Yosuke

AU - Ito, Takashi

AU - Nakayama, Tetsuo

PY - 2019/3/1

Y1 - 2019/3/1

N2 - We previously reported that recombinant measles virus expressing the respiratory syncytial virus (RSV) fusion protein (F), MVAIK/RSV/F, induced neutralizing antibodies against RSV, and those expressing RSV-NP (MVAIK/RSV/NP) and M2-1 (MVAIK/RSV/M2-1) induced RSV-specific CD8 + /IFN-γ + cells, but not neutralizing antibodies. In the present study, MVAIK/RSV/F and MVAIK/RSV/NP were simultaneously administered to cotton rats and immune responses and protective effects were compared with MVAIK/RSV/F alone. Sufficient neutralizing antibodies against RSV and RSV-specific CD8 + /IFN-γ + cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8 + /IFN-γ + increased in spleen cells obtained from the simultaneous immunization group in response to F and NP peptides. Higher numbers of CD8 + /IFN-γ + and CD4 + /IFN-γ + cells were detected in lung tissues from the simultaneous immunization group after the RSV challenge. No detectable RSV was recovered from lung homogenates in the immunized groups. Mild inflammatory reactions with the thickening of broncho-epithelial cells and the infiltration of inflammatory cells were observed in lung tissues obtained from cotton rats immunized with MVAIK/RSV/F alone after the RSV challenge. No inflammatory responses were observed after the RSV challenge in the simultaneous immunization groups. The present results indicate that combined administration with MVAIK/RSV/F and MVAIK/RSV/NP induces humoral and cellular immune responses and shows effective protection against RSV, suggesting the importance of cellular immunity.

AB - We previously reported that recombinant measles virus expressing the respiratory syncytial virus (RSV) fusion protein (F), MVAIK/RSV/F, induced neutralizing antibodies against RSV, and those expressing RSV-NP (MVAIK/RSV/NP) and M2-1 (MVAIK/RSV/M2-1) induced RSV-specific CD8 + /IFN-γ + cells, but not neutralizing antibodies. In the present study, MVAIK/RSV/F and MVAIK/RSV/NP were simultaneously administered to cotton rats and immune responses and protective effects were compared with MVAIK/RSV/F alone. Sufficient neutralizing antibodies against RSV and RSV-specific CD8 + /IFN-γ + cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8 + /IFN-γ + increased in spleen cells obtained from the simultaneous immunization group in response to F and NP peptides. Higher numbers of CD8 + /IFN-γ + and CD4 + /IFN-γ + cells were detected in lung tissues from the simultaneous immunization group after the RSV challenge. No detectable RSV was recovered from lung homogenates in the immunized groups. Mild inflammatory reactions with the thickening of broncho-epithelial cells and the infiltration of inflammatory cells were observed in lung tissues obtained from cotton rats immunized with MVAIK/RSV/F alone after the RSV challenge. No inflammatory responses were observed after the RSV challenge in the simultaneous immunization groups. The present results indicate that combined administration with MVAIK/RSV/F and MVAIK/RSV/NP induces humoral and cellular immune responses and shows effective protection against RSV, suggesting the importance of cellular immunity.

KW - Cotton rat

KW - F

KW - NP

KW - Recombinant virus

KW - RSV

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U2 - 10.3390/vaccines7010027

DO - 10.3390/vaccines7010027

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VL - 7

JO - Vaccines

JF - Vaccines

SN - 2076-393X

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