Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high‐performance liquid chromatography using A mixed‐mode column and an eight‐channel electrochemical detector

Fumiko Mashige, Yoshikazu Matsushima, Chizuko Miyata, Rika Yamada, Hideko Kanazawa, Ichiro Sakuma, Nobuharu Takai, Noriko Shinozuka, Akiyuki Ohkubo, Kazuhiko Nakahara

研究成果: Article査読

49 被引用数 (Scopus)

抄録

A high‐performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation‐exchange resin and a mixed‐mode (C18/cation‐exchange) column and determined by an eight‐channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3‐methoxy‐tyramine (3‐MT) and serotonin (5‐HT) were determined in 40 min with inter‐assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3‐MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3‐MT, 5‐HT were 99, 104, 104, 99, 102, 109 and 99% respectively.

本文言語English
ページ(範囲)221-225
ページ数5
ジャーナルBiomedical Chromatography
9
5
DOI
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

フィンガープリント

「Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high‐performance liquid chromatography using A mixed‐mode column and an eight‐channel electrochemical detector」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル