Simultaneous measurement of atrial natriuretic polypeptide (ANP) messenger RNA and ANP in rat heart - Evidence for a preferentially increased synthesis and secretion of ANP in left atrium of spontaneously hypertensive rats (SHR) -

Hiroshi Arai, Kazuwa Nakao, Yoshihiko Saito, Narito Morii, Akira Sugawara, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Masashi Mukoyama, Hiroaki Ohkubo, Shigetada Nakanishi, Hiroo Imura

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the rat heart were measured simultaneously. In Wistar rats, ANPmRNA of the same size (approximately 0.95 kbp) was detected in all four chambers of the rat heart. The ANPmRNA level was the highest in the right atrium, and the left atrial level was slightly lower than the right atrial level. Ventricular levels were more than two orders of magnitude lower than atrial levels. Tissue ANP concentrations of four chambers were roughly parallel to ANPmRNA levels. In spontaneously hypertensive rats (SHR) with the elevated plasma ANP level, the ANPmRNA level in the left atrium was substantially increased. The left/right ratio of atrial ANPmRNA level in SHR (150%) was higher than that in control Wistar Kyoto rats (WKY) (90%). In contrast, the left/right ratio of atrial ANP concentration was decreased in SHR (44%) compared with that in WKY (84%). The ratio of ANP to ANPmRNA levels in the left atrium of SHR was about three times smaller than that in the right atrium of SHR, and those in bilateral atria of WKY. These results indicate that the biosynthesis and secretion of ANP from the left atrium is preferentially increased in SHR. Thus, simultaneous determination of ANPmRNA and ANP levels is a refined strategy of investigation for the biosynthesis, storage and secretion of ANP.

本文言語English
ページ(範囲)239-245
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
148
1
DOI
出版ステータスPublished - 1987 10月 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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