Single-cell bioluminescence imaging of deep tissue in freely moving animals

Satoshi Iwano, Mayu Sugiyama, Hiroshi Hama, Akiya Watakabe, Naomi Hasegawa, Takahiro Kuchimaru, Kazumasa Z. Tanaka, Megumu Takahashi, Yoko Ishida, Junichi Hata, Satoshi Shimozono, Kana Namiki, Takashi Fukano, Masahiro Kiyama, Hideyuki Okano, Shinae Kizaka-Kondoh, Thomas J. McHugh, Tetsuo Yamamori, Hiroyuki Hioki, Shojiro MakiAtsushi Miyawaki

研究成果: Article査読

147 被引用数 (Scopus)

抄録

Bioluminescence is a natural light source based on luciferase catalysis of its substrate luciferin. We performed directed evolution on firefly luciferase using a red-shifted and highly deliverable luciferin analog to establish AkaBLI, an all-engineered bioluminescence in vivo imaging system. AkaBLI produced emissions in vivo that were brighter by a factor of 100 to 1000 than conventional systems, allowing noninvasive visualization of single cells deep inside freely moving animals. Single tumorigenic cells trapped in the mouse lung vasculature could be visualized. In the mouse brain, genetic labeling with neural activity sensors allowed tracking of small clusters of hippocampal neurons activated by novel environments. In a marmoset, we recorded video-rate bioluminescence from neurons in the striatum, a deep brain area, for more than 1 year. AkaBLI is therefore a bioengineered light source to spur unprecedented scientific, medical, and industrial applications.

本文言語English
ページ(範囲)935-939
ページ数5
ジャーナルScience
359
6378
DOI
出版ステータスPublished - 2018 2 23
外部発表はい

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Single-cell bioluminescence imaging of deep tissue in freely moving animals」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル