Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

Toshiro Sato, Robert G. Vries, Hugo J. Snippert, Marc Van De Wetering, Nick Barker, Daniel E. Stange, Johan H. Van Es, Arie Abo, Pekka Kujala, Peter J. Peters, Hans Clevers

研究成果: Article

2271 引用 (Scopus)

抄録

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5 + stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5 + stem cells can also initiate these crypt-villus organoids. Tracing experiments indicate that the Lgr5 + stem-cell hierarchy is maintained in organoids. We conclude that intestinal crypt-villus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.

元の言語English
ページ(範囲)262-265
ページ数4
ジャーナルNature
459
発行部数7244
DOI
出版物ステータスPublished - 2009 5 14
外部発表Yes

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Stem Cells
Organoids
Intestinal Mucosa
Mammals
In Vitro Techniques

ASJC Scopus subject areas

  • General

これを引用

Sato, T., Vries, R. G., Snippert, H. J., Van De Wetering, M., Barker, N., Stange, D. E., ... Clevers, H. (2009). Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature, 459(7244), 262-265. https://doi.org/10.1038/nature07935

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. / Sato, Toshiro; Vries, Robert G.; Snippert, Hugo J.; Van De Wetering, Marc; Barker, Nick; Stange, Daniel E.; Van Es, Johan H.; Abo, Arie; Kujala, Pekka; Peters, Peter J.; Clevers, Hans.

:: Nature, 巻 459, 番号 7244, 14.05.2009, p. 262-265.

研究成果: Article

Sato, T, Vries, RG, Snippert, HJ, Van De Wetering, M, Barker, N, Stange, DE, Van Es, JH, Abo, A, Kujala, P, Peters, PJ & Clevers, H 2009, 'Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche', Nature, 巻. 459, 番号 7244, pp. 262-265. https://doi.org/10.1038/nature07935
Sato T, Vries RG, Snippert HJ, Van De Wetering M, Barker N, Stange DE その他. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009 5 14;459(7244):262-265. https://doi.org/10.1038/nature07935
Sato, Toshiro ; Vries, Robert G. ; Snippert, Hugo J. ; Van De Wetering, Marc ; Barker, Nick ; Stange, Daniel E. ; Van Es, Johan H. ; Abo, Arie ; Kujala, Pekka ; Peters, Peter J. ; Clevers, Hans. / Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. :: Nature. 2009 ; 巻 459, 番号 7244. pp. 262-265.
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