Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells

Koichiro Homma, Masakatsu Sone, Daisuke Taura, Kenichi Yamahara, Yutaka Suzuki, Kazutoshi Takahashi, Takuhiro Sonoyama, Megumi Inuzuka, Yasutomo Fukunaga, Naohisa Tamura, Hiroshi Itoh, Shinya Yamanaka, Kazuwa Nakao

研究成果: Article査読

42 被引用数 (Scopus)

抄録

Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging.

本文言語English
ページ(範囲)42-47
ページ数6
ジャーナルAtherosclerosis
212
1
DOI
出版ステータスPublished - 2010 9月

ASJC Scopus subject areas

  • 循環器および心血管医学

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