Skeletal muscle regeneration after insulin-like growth factor I gene transfer by recombinant Sendai virus vector

A. Shiotani, M. Fukumura, M. Maeda, X. Hou, M. Inoue, T. Kanamori, S. Komaba, K. Washizawa, S. Fujikawa, T. Yamamoto, C. Kadono, K. Watabe, H. Fukuda, K. Saito, Y. Sakai, Y. Nagai, J. Kanzaki, M. Hasegawa

研究成果: Article査読

51 被引用数 (Scopus)

抄録

We scrutinized the applicability and efficacy of Sendai virus (SeV) vectors expressing either LacZ or human insulin-like growth factor-I (hIGF-I) in gene transfer into skeletal muscle. Seven days after the intramuscular injection of LacZ/SeV X-gal labeled myofibers were demonstrated in rat anterior tibialis muscle with/without bupivacaine treatment and the transgene expression persisted up to 1 month after injection. Recombinant hIGF-I was detected as a major protein species in culture supernatants of a neonatal rat myoblast cell line L6 and thus induced the cells to undergo myogenetic differentiation. The introduction of hIGF-I/SeV into the muscle showed a significant increase in regenerating and split myofibers which were indicative of hypertrophy, and also an increase in the total number of myofibers, in comparison to that seen in the LacZ/SeV-treated control muscle. These results demonstrate that SeV achieves high-level transgene expression in skeletal muscle, and that hIGF-I gene transfer using SeV vector may therefore have great potential in the treatment of neuromuscular disorders.

本文言語English
ページ(範囲)1043-1050
ページ数8
ジャーナルGene Therapy
8
14
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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