Small interfering RNA and gene silencing in transgenic mice and rats

Hidetoshi Hasuwa, Kazuhiro Kaseda, Thorbjorg Einarsdottir, Masaru Okabe

研究成果: Article査読

221 被引用数 (Scopus)

抄録

After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.

本文言語English
ページ(範囲)227-230
ページ数4
ジャーナルFEBS Letters
532
1-2
DOI
出版ステータスPublished - 2002 12 4
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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