Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy

Miyuki Murata, Kousuke Noda, Junichi Fukuhara, Atsuhiro Kanda, Satoru Kase, Wataru Saito, Yoko Ozawa, Satsuki Mochizuki, Shioko Kimura, Yukihiko Mashima, Yasunori Okada, Susumu Ishida

研究成果: Article

18 引用 (Scopus)

抄録

PURPOSE. Vascular adhesion protein (VAP)-1, a multifunctional molecule with adhesive and enzymatic properties, is expressed at the surface of vascular endothelial cells of mammals. It also exists as a soluble form (sVAP-1), which is implicated in oxidative stress via its enzymatic activity. This study explores a link between increased level of sVAP-1 and oxidative stress in proliferative diabetic retinopathy (PDR) with a focus on mechanistic components to form sVAP-1 by shedding from retinal endothelial cells. METHODS. Protein levels of sVAP-1 and N epsilon-(hexanoyl)lysine (HEL), an oxidative stress marker, in the vitreous samples from patients with PDR or non-PDR were measured by ELISA. The mechanism of VAP-1 shedding under diabetic condition, exposure to high glucose and/or inflammatory cytokines, was explored using cultured retinal capillary endothelial cells. RESULTS. Protein level of sVAP-1 was increased and correlated with HEL in the vitreous fluid of patients with PDR. Retinal capillary endothelial cells released sVAP-1 when stimulated with high glucose or inflammatory cytokines, such as TNF-α and IL-1β in vitro. Furthermore, matrix metalloproteinase-2 and -9, type IV collagenases, were the key molecules to mediate the protein cleavage of VAP-1 from retinal capillary endothelial cells. CONCLUSIONS. Our data for the first time provide evidence on the link between sVAP-1 and type IV collagenases in the pathogenesis of PDR.

元の言語English
ページ(範囲)4055-4062
ページ数8
ジャーナルInvestigative Ophthalmology and Visual Science
53
発行部数7
DOI
出版物ステータスPublished - 2012 6

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Diabetic Retinopathy
Blood Vessels
Endothelial Cells
Proteins
Oxidative Stress
Cytokines
Glucose
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Collagenases
Interleukin-1
Adhesives
Lysine
Mammals
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

これを引用

Murata, M., Noda, K., Fukuhara, J., Kanda, A., Kase, S., Saito, W., ... Ishida, S. (2012). Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy. Investigative Ophthalmology and Visual Science, 53(7), 4055-4062. https://doi.org/10.1167/iovs.12-9857

Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy. / Murata, Miyuki; Noda, Kousuke; Fukuhara, Junichi; Kanda, Atsuhiro; Kase, Satoru; Saito, Wataru; Ozawa, Yoko; Mochizuki, Satsuki; Kimura, Shioko; Mashima, Yukihiko; Okada, Yasunori; Ishida, Susumu.

:: Investigative Ophthalmology and Visual Science, 巻 53, 番号 7, 06.2012, p. 4055-4062.

研究成果: Article

Murata, M, Noda, K, Fukuhara, J, Kanda, A, Kase, S, Saito, W, Ozawa, Y, Mochizuki, S, Kimura, S, Mashima, Y, Okada, Y & Ishida, S 2012, 'Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy', Investigative Ophthalmology and Visual Science, 巻. 53, 番号 7, pp. 4055-4062. https://doi.org/10.1167/iovs.12-9857
Murata, Miyuki ; Noda, Kousuke ; Fukuhara, Junichi ; Kanda, Atsuhiro ; Kase, Satoru ; Saito, Wataru ; Ozawa, Yoko ; Mochizuki, Satsuki ; Kimura, Shioko ; Mashima, Yukihiko ; Okada, Yasunori ; Ishida, Susumu. / Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy. :: Investigative Ophthalmology and Visual Science. 2012 ; 巻 53, 番号 7. pp. 4055-4062.
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AU - Murata, Miyuki

AU - Noda, Kousuke

AU - Fukuhara, Junichi

AU - Kanda, Atsuhiro

AU - Kase, Satoru

AU - Saito, Wataru

AU - Ozawa, Yoko

AU - Mochizuki, Satsuki

AU - Kimura, Shioko

AU - Mashima, Yukihiko

AU - Okada, Yasunori

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