Solution exchange of droplet contacting lipid bilayer system

Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Hirotaka Sasaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

This paper describes a bilayer lipid membranes (BLMs) chip capable of solution exchange. In the previous report, we successfully reconstituted α-hemolysin (αHL) in BLMs using droplets contacting method with an array device. For drug screening, solution exchange is required to test the effect of inhibitors or promoters for proteins. In this study, we attempted to exchange the solution in a droplet using a microfluidic channel (Figure 1). We could successfully inject desired solution into the droplet forming BLMs and to withdraw the initial solution out of a droplet. The electrical signal of αHL with its some blockers which was injected later instead of the initial signal was successfully recorded. We consider that this method will enable more accurate and efficient drug screening system using artificially BLMs and reconstituted membrane protein.

本文言語English
ホスト出版物のタイトル2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
ページ882-885
ページ数4
DOI
出版ステータスPublished - 2012
イベント2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
継続期間: 2012 1 292012 2 2

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
国/地域France
CityParis
Period12/1/2912/2/2

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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