Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus

Kiyoshi Okada, Mari Takahashi, Taiichi Sakamoto, Gota Kawai, Kouji Nakamura, Akio Kanai

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus , whose loop-closing base pair is U:G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif, the conformational stabilities are significantly different to each other. It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.

本文言語English
ページ(範囲)383-395
ページ数13
ジャーナルNucleosides, Nucleotides and Nucleic Acids
25
4-6
DOI
出版ステータスPublished - 2006 6 1

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 遺伝学

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