TY - JOUR
T1 - Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus
AU - Okada, Kiyoshi
AU - Takahashi, Mari
AU - Sakamoto, Taiichi
AU - Kawai, Gota
AU - Nakamura, Kouji
AU - Kanai, Akio
N1 - Funding Information:
Received 14 December 2005; accepted 19 January 2006. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas (14035205) and, in part, by High Technology Research and from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by the RIKEN Structural Genomics/Proteomics Initiative (RSGI), the National Project on Protein Structural and Functional Analyses of MEXT. This article is dedicated to Professor Eiko Ohtsuka on the occasion of her 70th birthday. Address correspondence to Gota Kawai, Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-0016, Japan. E-mail: gkawai@sea.it-chiba.ac.jp
PY - 2006/6/1
Y1 - 2006/6/1
N2 - The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus , whose loop-closing base pair is U:G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif, the conformational stabilities are significantly different to each other. It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.
AB - The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus , whose loop-closing base pair is U:G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif, the conformational stabilities are significantly different to each other. It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.
KW - GNRR motif
KW - SRP
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U2 - 10.1080/15257770600683979
DO - 10.1080/15257770600683979
M3 - Article
C2 - 16838833
AN - SCOPUS:33747617315
SN - 1525-7770
VL - 25
SP - 383
EP - 395
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 4-6
ER -