Spatiotemporal quantification of subcellular ATP levels in a single HeLa cell during changes in morphology

Rika Suzuki, Kohji Hotta, Kotaro Oka

研究成果: Article査読

15 被引用数 (Scopus)

抄録

The quantitative relationship between change in cell shape and ATP consumption is an unsolved problem in cell biology. In this study, a simultaneous imaging and image processing analysis allowed us to observe and quantify these relationships under physiological conditions, for the first time. We focused on two marginal regions of cells: the microtubule-rich € lamella € and the actin-rich € peripheral structure €. Simultaneous imaging and correlation analysis revealed that microtubule dynamics cause lamellar shape change accompanying an increase in ATP level. Also, image processing and spatiotemporal quantification enabled to visualize a chronological change of the relationships between the protrusion length and ATP levels, and it suggested they are influencing each other. Furthermore, inhibition of microtubule dynamics diminished motility in the peripheral structure and the range of fluctuation of ATP level in the lamella. This work clearly demonstrates that cellular motility and morphology are regulated by ATP-related cooperative function between microtubule and actin dynamics.

本文言語English
論文番号16874
ジャーナルScientific reports
5
DOI
出版ステータスPublished - 2015 11 17

ASJC Scopus subject areas

  • 一般

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