Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin

Hidenori Sato, Kazuki Takeda, Koji Tani, Tomoya Hino, Tetsuji Okada, Masayoshi Nakasako, Nobuo Kamiya, Tsutomu Kouyama

研究成果: Article査読

73 被引用数 (Scopus)

抄録

In the purple membrane of Halobacterium salinarium, bacteriorhodopsin trimers are arranged in a hexagonal lattice. When purple membrane sheets are incubated at high temperature with neutral detergent, membrane vesicularization takes place, yielding inside-out vesicles with a diameter of 50 nm. The vesicular structure becomes unstable at low temperature, where successive fusion of the vesicles yields a crystal which is composed of stacked planar membranes. X-ray crystallographic analysis reveals that the bacteriorhodopsin trimers are arranged in a honeycomb lattice in each membrane layer and that neighbouring membranes orient in opposite directions. The native structure of the trimeric unit is preserved in the honeycomb lattice, irrespective of alterations in the in-plane orientation of the trimer. One phospholipid tightly bound to a crevice between monomers in the trimeric unit is suggested to act as a glue in the formation of the trimer.

本文言語English
ページ(範囲)1251-1256
ページ数6
ジャーナルActa Crystallographica Section D: Biological Crystallography
55
7
DOI
出版ステータスPublished - 1999 7月
外部発表はい

ASJC Scopus subject areas

  • 構造生物学

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