Specimen preparation for X-ray diffraction imaging experiments at cryogenic temperature

研究成果: Chapter

抄録

X-ray diffraction imaging (XDI) experiments at cryogenic temperatures allows us to analyze the internal structures of frozen-hydrated biological cells and cellular organelles. As biological specimens are easily degraded by drying, a humidity-controlled chamber is developed to maintain a moist atmosphere during specimen preparation. In the chamber, specimen particles are dispersed onto windows of thin silicon nitride membranes. Next, the particles are flash-cooled to a frozen-hydrated state using liquid ethane. This cryogenic method of specimen preparation allows us to harvest a large number of biological cells and isolated cellular organelles at desired periods in the cell cycle. Subsequently, the prepared specimens can be stored in liquid nitrogen until the use. In this chapter, the methods and devices developed for preparing frozen-hydrated biological specimens are described. In addition, the characteristics of frozen-hydrated specimens are described.

本文言語English
ホスト出版物のタイトルSpringer Series in Optical Sciences
出版社Springer Verlag
ページ107-124
ページ数18
DOI
出版ステータスPublished - 2018 1 1

出版物シリーズ

名前Springer Series in Optical Sciences
210
ISSN(印刷版)0342-4111
ISSN(電子版)1556-1534

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料

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