Split-and-contact device to form planar lipid bilayers

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

本文言語English
ホスト出版物のタイトルIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
ページ161-164
ページ数4
DOI
出版ステータスPublished - 2013
イベントIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
継続期間: 2013 1 202013 1 24

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
国/地域Taiwan, Province of China
CityTaipei
Period13/1/2013/1/24

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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