Stat3β inhibits γ-globin gene expression in erythroid cells

Heather A. Foley, Solomon F. Ofori-Acquah, Akihiko Yoshimura, Stuart Critz, B. Surendra Baliga, Betty S. Pace

研究成果: Article査読

24 被引用数 (Scopus)

抄録

We demonstrated previously γ-globin gene inhibition in K562 cells and primary erythroid progenitors treated with interleukin-6. Although several cis-acting elements have been identified in the globin promoters, the precise mechanism for cytokine-mediated globin gene regulation remains to be elucidated. In this report we demonstrate inhibitors of Stat3 phosphorylation abrogate interleukin-6-mediated γ gene silencing in erythroid cells. DNA-protein binding studies established Stat3 interaction in the 5′-untranslated γ-globin promoter region. Furthermore, co-transfection experiments with Stat3β demonstrate γ promoter inhibition in a concentration-dependent manner, which was significantly reversed when the cognate Stat3-binding site in the 5′-untranslated region was mutated. These studies establish a novel mechanism for γ gene silencing through the STAT signal transduction pathway.

本文言語English
ページ(範囲)16211-16219
ページ数9
ジャーナルJournal of Biological Chemistry
277
18
DOI
出版ステータスPublished - 2002 5 3
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Stat3β inhibits γ-globin gene expression in erythroid cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル