TY - JOUR
T1 - Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector
AU - Kanazawa, Hideko
AU - Tsubayashi, Akane
AU - Nagata, Yoshiko
AU - Matsushima, Yoshikazu
AU - Mori, Chiharu
AU - Kizu, Junko
AU - Higaki, Megumu
PY - 2002/3/1
Y1 - 2002/3/1
N2 - The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250×4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.
AB - The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250×4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.
KW - Circular dichroism detection
KW - Detection, LC
KW - Enantiomer separation
KW - Loxoprofens
KW - Nonsteriodal antiinflammatory drugs
KW - Profens
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U2 - 10.1016/S0021-9673(01)01312-7
DO - 10.1016/S0021-9673(01)01312-7
M3 - Article
C2 - 12831206
AN - SCOPUS:0036499518
SN - 0021-9673
VL - 948
SP - 303
EP - 308
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -