Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector

Hideko Kanazawa, Akane Tsubayashi, Yoshiko Nagata, Yoshikazu Matsushima, Chiharu Mori, Junko Kizu, Megumu Higaki

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250×4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.

本文言語English
ページ(範囲)303-308
ページ数6
ジャーナルJournal of Chromatography A
948
1-2
DOI
出版ステータスPublished - 2002 3月 1

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学

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