Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells

Masatoshi Kusuhara, Ken Yamaguchi, Masaru Kuranami, Ai Suzaki, Shiro Ishikawa, Hanlim Moon, Isamu Adachi, Shingo Hori, Shunnosuke Handa

研究成果: Article

16 引用 (Scopus)

抄録

Endothelin (ET) is a vasoconstrictor peptide originally isolated from vascular endothelial cells. Recent studies have revealed that ET has many biological functions including growth factor-like activity. The present study aims to clarify whether ET-1 possesses the ability to stimulate anchorage-independent cellular growth, an indicator of factors with transforming activity. We found that NRK 49F cells possess a large number of high-affinity ET-1 receptors; labeled 125I-ET-1 binding was displaced by unlabeled ET-2 in a similar dose response, but in the case of ET-3, 100-fold more was required. Specific 125I-ET-3 binding was undetectable in NRK 49F cells, indicating that ET receptors in NRK 49F cells are ET-1/ET-2 selective. NRK 49F is a cell line which is most commonly used to assay for anchorage-independent cellular growth. Therefore, we explored whether ETs promote anchorage-independent cellular growth in this cell line. ET-1 and ET-2 stimulated NRK colony formation dose dependently in the presence of 1 nM epidermal growth factor (EGF). In contrast, ET-3 did not have colony-stimulating ability. In the presence of EGF, the maximal effect of ET-1 was approximately 90% of that of transforming growth factor-β. Moreover, in the presence of maximal stimulating concentrations of EGF and transforming growth factor-β, ET-1 additionally induced colony formation. These results indicate that ET-1 and -2 possess transforming growth factor-like activity for NRK 49F cells. Since ET-1 and -2 increased intracellular calcium levels, this ion may participate in signal transduction pathways by which ET-1 and -2 promote colony formation.

元の言語English
ページ(範囲)3011-3014
ページ数4
ジャーナルCancer Research
52
発行部数11
出版物ステータスPublished - 1992 6 1

Fingerprint

Endothelins
Endothelin-1
Endothelin-2
Growth
Endothelin-3
Transforming Growth Factors
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Endothelin A Receptors
Cell Line
Endothelin Receptors
Vasoconstrictor Agents
Signal Transduction
Endothelial Cells
Ions
Calcium
Peptides

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

これを引用

Kusuhara, M., Yamaguchi, K., Kuranami, M., Suzaki, A., Ishikawa, S., Moon, H., ... Handa, S. (1992). Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells. Cancer Research, 52(11), 3011-3014.

Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells. / Kusuhara, Masatoshi; Yamaguchi, Ken; Kuranami, Masaru; Suzaki, Ai; Ishikawa, Shiro; Moon, Hanlim; Adachi, Isamu; Hori, Shingo; Handa, Shunnosuke.

:: Cancer Research, 巻 52, 番号 11, 01.06.1992, p. 3011-3014.

研究成果: Article

Kusuhara, M, Yamaguchi, K, Kuranami, M, Suzaki, A, Ishikawa, S, Moon, H, Adachi, I, Hori, S & Handa, S 1992, 'Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells', Cancer Research, 巻. 52, 番号 11, pp. 3011-3014.
Kusuhara M, Yamaguchi K, Kuranami M, Suzaki A, Ishikawa S, Moon H その他. Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells. Cancer Research. 1992 6 1;52(11):3011-3014.
Kusuhara, Masatoshi ; Yamaguchi, Ken ; Kuranami, Masaru ; Suzaki, Ai ; Ishikawa, Shiro ; Moon, Hanlim ; Adachi, Isamu ; Hori, Shingo ; Handa, Shunnosuke. / Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells. :: Cancer Research. 1992 ; 巻 52, 番号 11. pp. 3011-3014.
@article{89a028a8456b42f1b798fbe58bf96db4,
title = "Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells",
abstract = "Endothelin (ET) is a vasoconstrictor peptide originally isolated from vascular endothelial cells. Recent studies have revealed that ET has many biological functions including growth factor-like activity. The present study aims to clarify whether ET-1 possesses the ability to stimulate anchorage-independent cellular growth, an indicator of factors with transforming activity. We found that NRK 49F cells possess a large number of high-affinity ET-1 receptors; labeled 125I-ET-1 binding was displaced by unlabeled ET-2 in a similar dose response, but in the case of ET-3, 100-fold more was required. Specific 125I-ET-3 binding was undetectable in NRK 49F cells, indicating that ET receptors in NRK 49F cells are ET-1/ET-2 selective. NRK 49F is a cell line which is most commonly used to assay for anchorage-independent cellular growth. Therefore, we explored whether ETs promote anchorage-independent cellular growth in this cell line. ET-1 and ET-2 stimulated NRK colony formation dose dependently in the presence of 1 nM epidermal growth factor (EGF). In contrast, ET-3 did not have colony-stimulating ability. In the presence of EGF, the maximal effect of ET-1 was approximately 90{\%} of that of transforming growth factor-β. Moreover, in the presence of maximal stimulating concentrations of EGF and transforming growth factor-β, ET-1 additionally induced colony formation. These results indicate that ET-1 and -2 possess transforming growth factor-like activity for NRK 49F cells. Since ET-1 and -2 increased intracellular calcium levels, this ion may participate in signal transduction pathways by which ET-1 and -2 promote colony formation.",
author = "Masatoshi Kusuhara and Ken Yamaguchi and Masaru Kuranami and Ai Suzaki and Shiro Ishikawa and Hanlim Moon and Isamu Adachi and Shingo Hori and Shunnosuke Handa",
year = "1992",
month = "6",
day = "1",
language = "English",
volume = "52",
pages = "3011--3014",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "11",

}

TY - JOUR

T1 - Stimulation of anchorage-independent cell growth by endothelin in NRK 49F cells

AU - Kusuhara, Masatoshi

AU - Yamaguchi, Ken

AU - Kuranami, Masaru

AU - Suzaki, Ai

AU - Ishikawa, Shiro

AU - Moon, Hanlim

AU - Adachi, Isamu

AU - Hori, Shingo

AU - Handa, Shunnosuke

PY - 1992/6/1

Y1 - 1992/6/1

N2 - Endothelin (ET) is a vasoconstrictor peptide originally isolated from vascular endothelial cells. Recent studies have revealed that ET has many biological functions including growth factor-like activity. The present study aims to clarify whether ET-1 possesses the ability to stimulate anchorage-independent cellular growth, an indicator of factors with transforming activity. We found that NRK 49F cells possess a large number of high-affinity ET-1 receptors; labeled 125I-ET-1 binding was displaced by unlabeled ET-2 in a similar dose response, but in the case of ET-3, 100-fold more was required. Specific 125I-ET-3 binding was undetectable in NRK 49F cells, indicating that ET receptors in NRK 49F cells are ET-1/ET-2 selective. NRK 49F is a cell line which is most commonly used to assay for anchorage-independent cellular growth. Therefore, we explored whether ETs promote anchorage-independent cellular growth in this cell line. ET-1 and ET-2 stimulated NRK colony formation dose dependently in the presence of 1 nM epidermal growth factor (EGF). In contrast, ET-3 did not have colony-stimulating ability. In the presence of EGF, the maximal effect of ET-1 was approximately 90% of that of transforming growth factor-β. Moreover, in the presence of maximal stimulating concentrations of EGF and transforming growth factor-β, ET-1 additionally induced colony formation. These results indicate that ET-1 and -2 possess transforming growth factor-like activity for NRK 49F cells. Since ET-1 and -2 increased intracellular calcium levels, this ion may participate in signal transduction pathways by which ET-1 and -2 promote colony formation.

AB - Endothelin (ET) is a vasoconstrictor peptide originally isolated from vascular endothelial cells. Recent studies have revealed that ET has many biological functions including growth factor-like activity. The present study aims to clarify whether ET-1 possesses the ability to stimulate anchorage-independent cellular growth, an indicator of factors with transforming activity. We found that NRK 49F cells possess a large number of high-affinity ET-1 receptors; labeled 125I-ET-1 binding was displaced by unlabeled ET-2 in a similar dose response, but in the case of ET-3, 100-fold more was required. Specific 125I-ET-3 binding was undetectable in NRK 49F cells, indicating that ET receptors in NRK 49F cells are ET-1/ET-2 selective. NRK 49F is a cell line which is most commonly used to assay for anchorage-independent cellular growth. Therefore, we explored whether ETs promote anchorage-independent cellular growth in this cell line. ET-1 and ET-2 stimulated NRK colony formation dose dependently in the presence of 1 nM epidermal growth factor (EGF). In contrast, ET-3 did not have colony-stimulating ability. In the presence of EGF, the maximal effect of ET-1 was approximately 90% of that of transforming growth factor-β. Moreover, in the presence of maximal stimulating concentrations of EGF and transforming growth factor-β, ET-1 additionally induced colony formation. These results indicate that ET-1 and -2 possess transforming growth factor-like activity for NRK 49F cells. Since ET-1 and -2 increased intracellular calcium levels, this ion may participate in signal transduction pathways by which ET-1 and -2 promote colony formation.

UR - http://www.scopus.com/inward/record.url?scp=0026734649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026734649&partnerID=8YFLogxK

M3 - Article

C2 - 1317255

AN - SCOPUS:0026734649

VL - 52

SP - 3011

EP - 3014

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 11

ER -