Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co-culture of human osteosarcoma cells and human fibroblasts: Gelatinase-b production was stimulated via up-regulation of fibroblast growth factor (FGF) receptor

Takao Kurogi, Kazuki Nabeshima, Hiroaki Kataoka, Yasunori Okada, Masashi Koono

研究成果: Article査読

35 被引用数 (Scopus)

抄録

Co-cultures of human osteosarcoma Takase (OST) cells with various human fibroblasts derived from surgical specimens stimulated production of gelatinase B (92-kDa type-IV collagenase, MMP-9), tissue inhibitors of metalloproteinase (TIMP)-1, and TIMP-2 when compared to cultures of individual cells. The maximum stimulation of gelatinase-B production occurred at a cellular ratio of 1:1. Conditioned media from several fibroblast cultures stimulated OST cells to produce gelatinase B, TIMP-1 and TIMP-2, but not vice versa. Among various recombinant growth factors or cytokines, tumor necrosis factor (TNF)-α stimulated gelatinase-B production in cultures of OST cells alone, while recombinant basic fibroblast growth factor (bFGF) stimulated gelatinase-B production in co-cultures of OST cells with skin fibroblasts but not in individual cultures of each cell type. In the co-cultures, gelatinase-B production was inhibited by anti-bFGF monoclonal antibody (MAb), but not by anti-TNF-α MAb. This co-culture-specific stimulation of gelatinase-B production by bFGF was associated with increased expression of the FGF receptor in the co-culture.

本文言語English
ページ(範囲)82-90
ページ数9
ジャーナルInternational Journal of Cancer
66
1
DOI
出版ステータスPublished - 1996 3月 28

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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「Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co-culture of human osteosarcoma cells and human fibroblasts: Gelatinase-b production was stimulated via up-regulation of fibroblast growth factor (FGF) receptor」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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