Stimulation of myelin basic protein gene transcription by fyn tyrosine kinase for myelination

Hisashi Umemori, Yasunori Kadowaki, Kazushige Hirosawa, Yutaka Yoshida, Katsunori Hironaka, Hideyuki Okano, Tadashi Yamamoto

研究成果: Article査読

99 被引用数 (Scopus)

抄録

Myelin is synthesized about the time of birth. The Src-family tyrosine kinase Fyn is involved in the initial events of myelination. Fyn is present in myelin-forming cells and is activated through stimulation of celt surface receptors such as large myelin-associated glycoprotein (L-MAG). Here we show that Fyn stimulates transcription of the myelin basic protein (MBP) gene for myelination. MBP is a major component of the myelin membrane. In 4-week-old Fyn-deficient mice, MBP is significantly reduced, and electron microscopic analysis showed that myelination is delayed, compared with wild-type mice. The Fyn-deficient mice had thinner, more irregular myelin than the wild- type. We found that Fyn stimulates the promoter activity of the MBP gene by approximately sevenfold. The region responsible for the transactivation by Fyn is located between nucleotides -675 and -647 with respect to the transcription start site. Proteins binding to this region were found by gel shift study, and the binding activity correlates with Fyn activity during myelination. These results suggest that transactivation of the MBP gene by Fyn is important for myelination.

本文言語English
ページ(範囲)1393-1397
ページ数5
ジャーナルJournal of Neuroscience
19
4
DOI
出版ステータスPublished - 1999 2月 15
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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