Stimulation of the extrusion of protons and H+-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca2+ ions

Yoshiyuki Nakamura, Kunihiro Kasamo, Makato Sakata, Eiji Ohta

研究成果: Article査読

90 被引用数 (Scopus)

抄録

Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.

本文言語English
ページ(範囲)139-149
ページ数11
ジャーナルPlant and Cell Physiology
33
2
出版ステータスPublished - 1992 3 1

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

フィンガープリント 「Stimulation of the extrusion of protons and H<sup>+</sup>-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca<sup>2+</sup> ions」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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