Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice

Motohiro Kodama, Koichiro Asano, Tsuyoshi Oguma, Shizuko Kagawa, Katsuyoshi Tomomatsu, Misa Wakaki, Takahisa Takihara, Soichiro Ueda, Nao Ohmori, Hiromi Ogura, Jun Miyata, Kyuto Tanaka, Nobufumi Kamiishi, Koichi Fukunaga, Koichi Sayama, Eiji Ikeda, Taku Miyasho, Akitoshi Ishizaka

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Background: Allergen sensitization through a disrupted skin barrier appears to play a prominent role in the development of atopic diseases, including allergic asthma. The role of the genetic background in immunological and physiological phenotypes induced by epicutaneous sensitization is undetermined. Methods: BALB/c and C57BL/6 mice were sensitized either epicutaneously by patch application of ovalbumin (OVA) or systemically by intraperitoneal injection of OVA with alum before exposure to aerosolized OVA. The concentrations of OVA-specific immunoglobulin in serum and cytokines in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The severity of airway inflammation was evaluated by cell counts in BALF, and bronchial responsiveness to methacholine was measured by the flexiVent system. Results: The production of OVA-specific IgG1 and IgE was greater in the epicutaneously sensitized BALB/c than C57BL/6 mice. In contrast, both eosinophilic airway inflammation and bronchial responsiveness to methacholine were more prominent in the C57BL/6 than in the BALB/c mice. The concentrations of interleukin-4 increased significantly in the BALF from C57BL/6 mice only. No between-strain differences were observed after intraperitoneal sensitization. Conclusions: The C57BL/6 mouse is a more appropriate model than the BALB/c mouse to study the relationship between skin barrier dysfunction and the pathogenesis of allergic asthma.

本文言語English
ページ(範囲)67-74
ページ数8
ジャーナルInternational Archives of Allergy and Immunology
152
SUPPL. 1
DOI
出版ステータスPublished - 2010 6

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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