Structural alterations of the p53 gene in human leukemias

Hiroshi Miwa, Kenkichi Kita, Hideyuki Saya, Akira Otsuji, Masahiro Masuya, Kazuhiro Nishii, Norihisa Morita, Nobuyuki Takakura, Koushi Ohishi, Kaori Nasu, Kazunori Nakase, Shigeru Shirakawa

研究成果: Article

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We have investigated alterations of the p53 gene in human leukemias by polymerase chain reaction-mediated restriction fragment length polymorphism analysis. This method detects the codon 72 polymorphism of the p53 gene, allowing identification of loss of heterozygosity (LOH) of the p53 gene. In this study, at least two specimens were obtained from each patient to compare the allele status at different points of clinical course. Of 21 cases examined 7 were heterozygous at this polymorphic site and were evaluable for LOH study. Only one patient of Philadelphia chromosomepositive acute lymphoblastic leukemia (ALL) lost the heterozygosity in the specimen of her last relapse. Leukemic cells from her repeated relapses including the last one revealed to have the clonally rearranged immunoglobulin H chain gene of the same size, indicating that alteration of the p53 gene in this patient might account for the lethal relapse as a clonal evolution event. Northern-blot analysis of the p53 gene showed that one Gase of CD7(+) ALL had altered p53 mRNA. Overall, it is demonstrated that alterations of the p53 gene might be involved in the pathogenesis or progression of at least some human leukemias, although the alterations in leukemias seemed to be not as frequent as in solid tumors.

元の言語English
ページ(範囲)1105-1112
ページ数8
ジャーナルLeukemia Research
16
発行部数11
DOI
出版物ステータスPublished - 1992 11
外部発表Yes

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

フィンガープリント Structural alterations of the p53 gene in human leukemias' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Miwa, H., Kita, K., Saya, H., Otsuji, A., Masuya, M., Nishii, K., Morita, N., Takakura, N., Ohishi, K., Nasu, K., Nakase, K., & Shirakawa, S. (1992). Structural alterations of the p53 gene in human leukemias. Leukemia Research, 16(11), 1105-1112. https://doi.org/10.1016/0145-2126(92)90049-D