Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS

Xingyu Zhu, Kenichi Hatanaka, Tatsuya Yamagata, Toshinori Sato

研究成果: Article査読

5 被引用数 (Scopus)

抄録

A glycosphingolipid analogue (12-azidododecyl β-lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE-ESI-MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12-azidododecyl b-lactoside (Lac-C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac-C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CEESI-MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity.

本文言語English
ページ(範囲)3519-3526
ページ数8
ジャーナルELECTROPHORESIS
30
20
DOI
出版ステータスPublished - 2009 12 2

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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