Structural basis for the inhibition of voltage-dependent K+ channel by gating modifier toxin

Shin Ichiro Ozawa, Tomomi Kimura, Tomohiro Nozaki, Hitomi Harada, Ichio Shimada, Masanori Osawa

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Voltage-dependent K+ (Kv) channels play crucial roles in nerve and muscle action potentials. Voltagesensing domains (VSDs) of Kv channels sense changes in the transmembrane potential, regulating the K+-permeability across the membrane. Gating modifier toxins, which have been used for the functional analyses of Kv channels, inhibit Kv channels by binding to VSD. However, the structural basis for the inhibition remains elusive. Here, fluorescence and NMR analyses of the interaction between VSD derived from KvAP channel and its gating modifier toxin, VSTx1, indicate that VSTx1 recognizes VSD under depolarized condition. We identified the VSD-binding residues of VSTx1 and their proximal residues of VSD by the cross-saturation (CS) and amino acid selective CS experiments, which enabled to build a docking model of the complex. These results provide structural basis for the specific binding and inhibition of Kv channels by gating modifier toxins.

本文言語English
論文番号14226
ジャーナルScientific reports
5
DOI
出版ステータスPublished - 2015 9月 18

ASJC Scopus subject areas

  • 一般

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