Structural characterization of y29f mutant of thermoglobin from a hyperthermophilic bacterium aquifex aeolicus

Norifumi Muraki, Kouta Takeda, Dayeon Nam, Megumi Muraki, Shigetoshi Aono

研究成果: Article査読

抄録

We have determined the crystal structure of thermoglobin (AaTgb) from a hyperthermophilic bacterium Aquifex aeolicus. Tyrosine and glutamine at the B10 and E7 position, respectively, are conserved in AaTgb as are the case of single domain hemoglobins (sdHbs). While the binding affinity of O2 or CO is affected by the replacement of Tyr29 in the distal heme pocket, wild type and Y29F variant of AaTgb show a similar binding affinity of imidazole.

本文言語English
論文番号200879
ジャーナルChemistry Letters
50
4
DOI
出版ステータスPublished - 2021
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)

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