@article{bd0e768572474e87877c150e6292f663,
title = "Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1",
abstract = "mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.",
keywords = "Circular dichroism, Elav, Musashi, Northwestern blotting, Secondary structure, U1 snRNP A, α-helix, β-sheet",
author = "Yasuyuki Kurihara and Takashi Nagata and Takao Imai and Ado Hiwatashi and Masataka Horiuchi and Sakakibara, {Shin ichi} and Masato Katahira and Hideyuki Okano and Seiichi Uesugi",
note = "Funding Information: The authors thank Mr. M. Miyauchi and M. Ohtsu for their skillful technical assistance, and Dr. M. Okabe for a kind help in preparing the manuscript. This work was supported by grants from the Japanese Ministry of Education, Science and Culture to Y.K., S.S., M.K., H.O. and S.U. and partly by the Okano Project of TARA, University of Tsukuba and Uehara Foundation and Proposal-Based Advanced Industrial Technology R&D Program to M.K. and S.U.. S.S. is a research fellow of the Japan Society for the Promotion of Science.",
year = "1997",
month = feb,
day = "20",
doi = "10.1016/S0378-1119(96)00673-7",
language = "English",
volume = "186",
pages = "21--27",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",
}