Structure of acidic polysaccharide from cell wall of Propionibacterium acnes strain C7

Masato Nagaoka, Kei Ichi Kamisango, Hideji Fujii, Kei Ichi Uchikawa, Isao Sekikawa, Ichiro Azuma

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The structure of polysaccharide prepared by lysozyme digestion from the cell wall of Propionibacterium acnes strain C7 was examined. The polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminoman-nuronic acid in a molar ratio of 1: 1: 0.3: 1: 2. By Smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideox-ymannuronic acid [Man(NAc)2A] by means of 1H-NMR and 13C-NMR spectro-scopic analyses. The results of analyses involving methylation and partial acid hydrolysis led to the conclusion that the polysaccharide has the repeating unit↑ 6)Gal(αl ↑4)Man(NAc)2A(β1 -6)Glc(αl ↑4) Man(NAc)2A(β1 ↑3)GalNAc(β1↑. In addition, a portion of the galactose residues were substituted at C-4 by al ↑2 linked mannotriose.

本文言語English
ページ(範囲)1669-1678
ページ数10
ジャーナルJournal of biochemistry
97
6
DOI
出版ステータスPublished - 1985 6月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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