The structure of polysaccharide prepared by lysozyme digestion from the cell wall of Propionibacterium acnes strain C7 was examined. The polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminoman-nuronic acid in a molar ratio of 1: 1: 0.3: 1: 2. By Smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideox-ymannuronic acid [Man(NAc)2A] by means of 1H-NMR and 13C-NMR spectro-scopic analyses. The results of analyses involving methylation and partial acid hydrolysis led to the conclusion that the polysaccharide has the repeating unit↑ 6)Gal(αl ↑4)Man(NAc)2A(β1 -6)Glc(αl ↑4) Man(NAc)2A(β1 ↑3)GalNAc(β1↑. In addition, a portion of the galactose residues were substituted at C-4 by al ↑2 linked mannotriose.
|ジャーナル||Journal of biochemistry|
|出版ステータス||Published - 1985 6月|
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