Structures and intracellular delivery of pDNA/sugar-modified chitosan complexes

Toshinori Sato, Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie

研究成果: Paper査読

抄録

Chitosan is a useful nonviral vector for gene delivery. In order to make a pDNA/chitosan complex specific to hepatocytes and macrophages, lactose-or mannose-modified chitosan (lac-chitosan or man-chitosan) was synthesized. 8%lac-chitosan showed excellent DNA-binding ability, good protection of DNA from nuclease, and the suppression of self-aggregation and serum-induced aggregation. The cell transfection efficiency of the pDNA/8%lac-chitosan complexes was greater for HepG2 cells. Observations with a confocal laser scanning microscope indicated that the pDNA/lac-chitosan complexes were delivered to the late endosome and have the advantage of delivering DNA to the perinuclear region. The cellular uptake of pDNA/man-chitosan complexes to mause peritoneal macrophages was observed. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes.

本文言語English
ページ5371-5372
ページ数2
出版ステータスPublished - 2006 12 1
イベント55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
継続期間: 2006 9 202006 9 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
国/地域Japan
CityToyama
Period06/9/2006/9/22

ASJC Scopus subject areas

  • 工学(全般)

フィンガープリント

「Structures and intracellular delivery of pDNA/sugar-modified chitosan complexes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル