Chitosan is a useful nonviral vector for gene delivery. In order to make a pDNA/chitosan complex specific to hepatocytes and macrophages, lactose-or mannose-modified chitosan (lac-chitosan or man-chitosan) was synthesized. 8%lac-chitosan showed excellent DNA-binding ability, good protection of DNA from nuclease, and the suppression of self-aggregation and serum-induced aggregation. The cell transfection efficiency of the pDNA/8%lac-chitosan complexes was greater for HepG2 cells. Observations with a confocal laser scanning microscope indicated that the pDNA/lac-chitosan complexes were delivered to the late endosome and have the advantage of delivering DNA to the perinuclear region. The cellular uptake of pDNA/man-chitosan complexes to mause peritoneal macrophages was observed. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes.
|出版ステータス||Published - 2006 12月 1|
|イベント||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
継続期間: 2006 9月 20 → 2006 9月 22
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||06/9/20 → 06/9/22|
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