Study for the Ho:YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system

Masato Sato, Miya Ishihara, Tsunenori Arai, Takashi Asazuma, Toshiyuki Kikuchi, Makoto Kikuchi, Kyosuke Fujikawa

研究成果: Conference article

1 引用 (Scopus)

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The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 μm, pulse width: about 200μs) was delivered through a 200μm-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 - 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54J (closed to practically used condition), but in 27J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser - disc cell interactions.

元の言語English
ページ(範囲)216-221
ページ数6
ジャーナルProceedings of SPIE - The International Society for Optical Engineering
3914
出版物ステータスPublished - 2000 1 1
イベントLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA
継続期間: 2000 1 222000 1 27

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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