Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe

Takeshi Kuroda, Kiyotake Suenaga, Akira Sakakura, Tomohisa Handa, Kazuhito Okamoto, Hideo Kigoshi

研究成果: Article査読

45 被引用数 (Scopus)

抄録

The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.

本文言語English
ページ(範囲)524-529
ページ数6
ジャーナルBioconjugate Chemistry
17
2
DOI
出版ステータスPublished - 2006 3 1
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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