Sugar microanalysis by HPLC with benzoylation: Improvement via introduction of a C-8 cartridge and a high efficiency ODS column

Michiko Miyagi, Hirokazu Yokoyama, Toshifumi Hibi

研究成果: Article査読

18 被引用数 (Scopus)

抄録

An HPLC protocol for sugar microanalysis based on the formation of ultraviolet-absorbing benzoyl chloride derivatives was improved. Here, samples were prepared with a C-8 cartridge and analyzed with a high efficiency ODS column, in which porous spherical silica particles 3 μm in diameter were packed. These devices allowed us to simultaneously quantify multiple sugars and sugar alcohols up to 10 ng/ml and to provide satisfactory separations of some sugars, such as fructose and myo-inositol and sorbitol and mannitol. This protocol, which does not require special apparatuses, should become a powerful tool in sugar research.

本文言語English
ページ(範囲)286-290
ページ数5
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
854
1-2
DOI
出版ステータスPublished - 2007 7 1

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 細胞生物学

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