Sulfatide accumulation in the dystrophic terminals of gracile axonal dystrophy mice: Lipid analysis using matrix-assisted laser desorption/ionization imaging mass spectrometry

Sayoko Onishi, Yoshiki Tatsumi, Keiji Wada, Hyun Jeong Yang, Yuki Sugiura, Mitsutoshi Setou, Hiroo Yoshikawa

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The gracile axonal dystrophy (gad) mutation in Uch-l1, the gene encoding the ubiquitin carboxy-terminal hydrolase isozyme L1 (UCH-L1), causes selective dying back degeneration of dorsal root ganglion neuron in the medulla oblongata along with progressive sensory-motor ataxia. Axonal spheroids are observed within degenerating axons, and their contents may illuminate the pathogenic mechanisms leading to neurodegeneration in gad mice. To analyze changes in negatively charged lipid molecules in dystrophic axons of gad mice, we performed matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS), electron microscopy, and fluorescence immunohistochemistry on tissue sections from gad and wild-type mouse medulla. MALDI-IMS revealed that m/z 806.68 and 822.68 molecules, assigned to sulfatide (ST) C18:0 and ST C18:0(OH), respectively, were concentrated in the dorsomedial medulla. This spatial distribution overlapped significantly with that of axonal spheroids. Immunostaining revealed that spheroids accumulated myelin and lymphocyte protein, a known ST binding protein. Sulfatides with short-chain fatty acids (C16-C20) are generally localized in intracellular vesicles; therefore, ST C18:0 accumulation may reflect intracellular vesicle aggregation within spheroids. Ubiquitin system disruption apparently alters lipid metabolism, membrane organization, protein turnover, and axonal transport. Changes in membrane organization, particularly STs within lipid rafts, may disrupt cellular signaling pathways necessary for neuronal viability.

本文言語English
ページ(範囲)160-165
ページ数6
ジャーナルMedical Molecular Morphology
46
3
DOI
出版ステータスPublished - 2013 9月

ASJC Scopus subject areas

  • 病理学および法医学
  • 分子生物学

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