Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells

Tohru Mizushima, Shinji Tsutsumi, Kazuhito Rokutan, Tomofusa Tsuchiya

研究成果: Article

43 引用 (Scopus)

抄録

The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose- dependent manner. The maximum effect was achieved at 10-6 M, at which concentration the drug was previously shown to induce heat-shock proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.

元の言語English
ページ(範囲)510-514
ページ数5
ジャーナルDigestive Diseases and Sciences
44
発行部数3
出版物ステータスPublished - 1999
外部発表Yes

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geranylgeranylacetone
DNA Fragmentation
Stomach
Guinea Pigs
Ethanol
Heat-Shock Proteins
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells. / Mizushima, Tohru; Tsutsumi, Shinji; Rokutan, Kazuhito; Tsuchiya, Tomofusa.

:: Digestive Diseases and Sciences, 巻 44, 番号 3, 1999, p. 510-514.

研究成果: Article

Mizushima, Tohru ; Tsutsumi, Shinji ; Rokutan, Kazuhito ; Tsuchiya, Tomofusa. / Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells. :: Digestive Diseases and Sciences. 1999 ; 巻 44, 番号 3. pp. 510-514.
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