Suppression of heat shock protein 27 expression promotes 5-fluorouracil sensitivity in colon cancer cells in a xenograft model

Ryohei Hayashi, Yoshiyuki Ishii, Hiroki Ochiai, Atsushi Matsunaga, Takashi Endo, Hirotoshi Hasegawa, Yuko Kitagawa

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The present study investigates whether the expression levels of heat shock protein 27 (HSP27) in colon cancer cells are associated with 5-fluorouracil (5-FU) sensitivity in a xenograft model, as well as the mechanism responsible for regulating 5-FU sensitivity. HCT116 cells which have a high expression of HSP27 were stably transfected with specific short hairpin RNA (shRNA) in order to suppress HSP27 expression. The association between HSP27 protein expression levels and 5-FU sensitivity was evaluated in a mouse xenograft model. The mRNA expression of 5-FU metabolic enzymes and cell apoptosis were also analyzed in the transfected cells. The suppression of HSP27 protein expression led to enhanced 5-FU sensitivity. The mRNA expression levels of dihydropyrimidine dehydrogenase and orotate phosphoribosyltransferase, but not those of thymidylate synthase, and the number of apoptotic cells increased in the transfected cells after 5-FU exposure. In conclusion, the suppression of HSP27 expression in colon cancer cells may promote 5-FU sensitivity by inducing apoptosis, despite the acceleration in 5-FU metabolism.

本文言語English
ページ(範囲)1269-1274
ページ数6
ジャーナルOncology reports
28
4
DOI
出版ステータスPublished - 2012 10月

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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