Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering

Makiko Kawada, Asako Takeuchi, Keiji Fujimoto

研究成果: Paper査読

抄録

Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

本文言語English
ページ数1
出版ステータスPublished - 2005 12月 1
イベント54th SPSJ Annual Meeting 2005 - Yokohama, Japan
継続期間: 2005 5月 252005 5月 27

Other

Other54th SPSJ Annual Meeting 2005
国/地域Japan
CityYokohama
Period05/5/2505/5/27

ASJC Scopus subject areas

  • 工学(全般)

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