Symmetric covalent linkage of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) results in novel derivatives with increased inhibitory activities against calcium/calmodulin complex

Hisayuki Yokokura, Masanori Osawa, Tsutomu Inoue, Isao Umezawa, Yasuhito Naito, Mitsuhiko Ikura, Hiroyoshi Hidaka

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A useful calmodulin (CaM) antagonist, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), was invented by Hidaka et al. in 1978. Here, we have designed new CaM antagonists on the basis of the three-dimensional structure of Ca2+/CaM complexed with W-7. Eleven new compounds all share a similar architecture, in which two W-7 molecules are linked between their aminohexyl termini by a linker with different functionalities. A wide range of inhibitory activities against Ca2+/CaM-dependent protein kinase I (CaM kinase I) has been observed with these self-crosslinked W-7 analogs, (W-7)2. In vitro competitive CaM kinase I assays using CaM kinase I and nuclear magnetic resonance studies indicated that one (W-7)2 molecule binds to one CaM molecule as expected, with the two chloronaphthalene rings of (W-7)2 being anchored separately to the N- and C-terminal hydrophobic pockets of Ca2+/CaM. The most potent compound, N,N'-bis[6-(5-chloro-1-naphthalenesulfonyl)-amino-1-hexyl]-p-xylene-diamine ((W-7)2- 10), inhibits CaM kinase I activity at an IC50 value of 0.23 μM; about 75 times more effectively than W-7. The length and basicity of the linker sequence in (W-7)2 significantly contribute to inhibitory activity. The present study opens an avenue for developing powerful CaM antagonists that could be used at low doses in vivo.

本文言語English
ページ(範囲)203-216
ページ数14
ジャーナルDrug Design and Discovery
16
3
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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