Synaptotagmin IV is present at the Golgi and distal parts of neurites

Keiji Ibata, Mitsunori Fukuda, Tomohiro Hamada, Hiroyuki Kabayama, Katsuhiko Mikoshiba

研究成果: Article査読

65 被引用数 (Scopus)

抄録

Synaptotagmin IV (SytIV) is an immediate early gene induced by membrane depolarization in PC12 cells and in rat brain. However, little is known about the function of SytlV or the functional relationship between SytlV and SytI, because SytlV has yet to be localized. Here we show that SytlV was localized at the Golgi and distal part of neurites in nerve growth factor- differentiated PC12 cells and cultured hippocampal neurons by immunocytochemistry using an isoform-specific antibody (anti-SytIV). These SytlV signals were not colocalized well with SytI signals. Upon membrane depolarization, SytlV signals were increased at both the Golgi and distal part of neurites within several hours in both types of cells. We further show that the increase of SytlV protein levels results from protein kinase A- dependent gene up-regulation. In hippocampal neurons, SytlV was developmentally regulated. These results suggest that SytlV may play a role at the Golgi and tips of neurites during development and synaptic plasticity.

本文言語English
ページ(範囲)518-526
ページ数9
ジャーナルJournal of Neurochemistry
74
2
DOI
出版ステータスPublished - 2000 1月 29

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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