Synthesis and characterization of 1,2-dimyristoylamido-1,2-deoxyphosphatidylcholine as an artificial boundary lipid

Junzo Sunamoto, Mitsuaki Goto, Kiyoshi Iwamoto, Hiroki Kondo, Toshinori Sato

研究成果: Article査読

48 被引用数 (Scopus)

抄録

The synthesis and characterization of an artificial boundary lipid, 1,2-dimyristoylamido-1,2-deoxyphosphatidylcholine (DDPC), are described. DDPC has two amide bonds instead of ester bonds of regular lecithins such as 1,2-dimyristoylphosphatidylcholine (DMPC). In differential scanning calorimetry (DSC) measurements, DDPC gave two endothermic peaks: one was at 18.0°C (ΔH = 10.74 kJ·mol-1) and the other at 23.0°C (ΔH = 12.91 kJ·mol-1). The former peak was sharp and considered to be the phase transition of the hydrocarbon region, while the latter was assigned to the melt of the hydrogen-belt formed by the amide groups of DDPC. Addition of DDPC to DMPC made the DMPC membrane less fluid in the region close to the surface, and significantly increased the reconstitution efficiency of glycophorin into the membrane. This effect of DDPC was much larger than that of naturally occurring lipid, sphingomyelin.

本文言語English
ページ(範囲)209-219
ページ数11
ジャーナルBBA - Biomembranes
1024
2
DOI
出版ステータスPublished - 1990 5月 24
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 細胞生物学

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