Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko, Higashizaki Takako, Ozawa Fumiko, Hirai Keiko, Nishizawa Masafumi, Tokiinaga Masao, Tsukui Hiroko, Hatanaka Hiroshi, Hishinuma Fumio

研究成果: Article査読

33 被引用数 (Scopus)

抄録

The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

本文言語English
ページ(範囲)65-74
ページ数10
ジャーナルGene
83
1
DOI
出版ステータスPublished - 1989 11 15
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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