Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko; Higashizaki Takako; Ozawa Fumiko; Hirai Keiko; Nishizawa Masafumi; Tokiinaga Masao; Tsukui Hiroko; Hatanaka Hiroshi; Hishinuma Fumio

doi:10.1016/0378-1119(89)90404-6

Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko, Higashizaki Takako, Ozawa Fumiko, Hirai Keiko, Nishizawa Masafumi, Tokiinaga Masao, Tsukui Hiroko, Hatanaka Hiroshi, Hishinuma Fumio

研究成果: Article › 査読

33 被引用数 (Scopus)

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The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

本文言語	English
ページ（範囲）	65-74
ページ数	10
ジャーナル	Gene
巻	83
号	1
DOI	https://doi.org/10.1016/0378-1119(89)90404-6
出版ステータス	Published - 1989 11月 15
外部発表	はい

ASJC Scopus subject areas

遺伝学

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10.1016/0378-1119(89)90404-6

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abstract = "The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.",

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AU - Eiko, Kanaya

AU - Takako, Higashizaki

AU - Fumiko, Ozawa

AU - Keiko, Hirai

AU - Masafumi, Nishizawa

AU - Masao, Tokiinaga

AU - Hiroko, Tsukui

AU - Hiroshi, Hatanaka

AU - Fumio, Hishinuma

PY - 1989/11/15

Y1 - 1989/11/15

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AB - The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

KW - (Recombinant DNA

KW - expression in yeast

KW - neurite outgrowth)

KW - pheochromocytoma cell

KW - pre-pro sequence

KW - processing of precursor protein

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Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

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